Time-lapse video of Alox5+/+ (green) and Alox5−/− (red) bone marrow–derived eosinophils and a C. elegans dauer larva (blue) over the course of 60 min. Images were acquired once every 60 s and are displayed at a frame rate of 10/s. Fluorescent eosinophils and invisible nonfluorescent Alox5+/+ eosinophils make up 30% and 70% of the total cells, respectively. Stills from this video are shown in Fig. 3. Results represent two independent experiments. Bar, 50 µm.
Michael L. Patnode, Jennifer K. Bando, Matthew F. Krummel, Richard M. Locksley, and Steven D. Rosen