NETs in vivo. Time-lapse movie of the IVC 4 h after flow reduction acquired by intravital two-photon microscopy. Extracellular DNA was visualized by Sytox orange (red; arrow) originating from neutrophils (green, labeled with an anti–Ly6G-FITC antibody; arrowhead) attached to the venous vessel wall (blue; frame rate: 10 frames/s).