An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning. The UtrCH-GFP (green) was used to label cortical changes during spindle migration. Kymograph of the UtrCH-GFP was provided as in Fig. S2 L. The video again shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex. Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.
Kexi Yi, Boris Rubinstein, Jay R. Unruh, Fengli Guo, Brian D. Slaughter, and Rong Li