An oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning. The UtrCH-GFP (green) was used to label cortical changes during spindle migration. Kymograph of the UtrCH-GFP was provided as in Fig. S2 L. The video again shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex. Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.
